Predicted complete ORFs that are identical to cDNA clones but have no BLAST hits
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7.1 years ago
nancydong20 ▴ 130

Hello!

I assembled a number of transcripts from a non-model organism and used TransDecoder to predict ones with complete ORFs. I see that a number of the transcripts containing complete ORFs don't have a hit in protein or RNA BLAST databases, but are identical to entries in the BLAST EST database from the same species. What are these transcripts? Are they protein-coding genes that don't have homology with known genes, or are they pseudogenes? Or both?

Thank you very much!

RNA-Seq • 1.1k views
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