convert illumina to fastq Sanger

Question

[aymenbenchaalia1]

Hello, please, what is the line command that allows you to convert an illuminated file into fastq snager thanks for your help

[Sej Modha]

Have you tried tools provided here: Illumina-Fastq To Sanger-Fastq Conversion

[aymenbenchaalia1]

I want to convert an Illuminat type file to fastq Sanger i need the command line

[Daniel]

Please explain what you are trying to achieve and why, so that we can help you, as this request is confusing. Do you have a bcl file?

[aymenbenchaalia1]

I want to convert an Illuminat type file to fastq Sanger i need the command line

[WouterDeCoster]

Repeating the question does not count as explaining. Please elaborate.

Answer 1

[Sej Modha]

Please try the seqtk solution provided on this post: Illumina-Fastq To Sanger-Fastq Conversion

seqtk seq -Q64 -V  Read1.fastq > Read1.sanger.fastq

PS: It is good practice to ensure that a similar question doesn't exist or provided solutions don't work before a question is posted here.

Answer 2

[GenoMax]

You can also use reformat.sh from BBMap suite as follows: reformat.sh in=your_fastq.gz out=sanger_fastq.gz qout=33 (adjust file names as needed, use in1= in2= out1= out2= for PE data.