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7.1 years ago
bioinfo_ga
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70
I have used tuxedo pipeline for rnaseq analysis of mouse samples. Reference and gtf(mm10) were downloaded from ucsc. In my cuffdiff results for one gene GAD1 i am observing gene elongation as its original coordinates are 70562129-70602014 and the coordinates observed in cuffdiff are 70489940-70563357. What could be the reason?