Entering edit mode
7.1 years ago
cupplesa
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0
I have generated a diamond file by aligning a set of genes to a set of shotgun sequencing data.
Could you recommend a software to analyze the data? The file is too big to manipulate in excel.
I have tried Megan, but this seems to use only KEGG, SEED etc, not a set of predefined genes.
You need to add additional clarification as to what exactly you are doing.
Are you referring to DIAMOND that is used as a BLAST+ replacement to do
blastxsearches? Why were you aligning known genes to shotgun sequences (rather than other way around)? What kind of known genes are these (NCBI nr)?Hey, guys. I am having the same issue. I have aligned a set of shotgun sequencing data against nearly 600 genes using DIAMOND blastx. I want to know which genes are present and which are absent in my shotgun sequencing data! Do you have any recommendation how can I do it?