Conservation of amino acid

Question

[Tania]

Hi all

How can I check if a given amino acid is conserved among species and get a table like this showing conservation? thank you

                 Helix A          Helix B
Cyp40      273-306 LSCVLNIGACKLKMSNWQGAIDSCLEALELDPSN
FKBP51     317-350 LAAFLNLAMCYLKLREYTKAVECCDKALGLDSAN
FKBP52     319-352 LASHLNLAMCHLKLQAFSAAIESCNKALELDSNN
PP5        062-095 AIYYGNRSLAYLRTECYGYALGDATRAIELDKKY
Hop TPR1   038-071 HVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDW
Hop TPR2A  259-292 MTYITNQAAVYFEKGDYNKCRELCEKAIEVGREN

Answer 1

[GenoMax]

By doing multiple sequence alignments and then looking for conserved residues at certain positions.

[Tania]

So is there a tool that I can use to do this? I tried but doesn't work https://www.ncbi.nlm.nih.gov/homologene?cmd=Retrieve&dopt=MultipleAlignment&list_uids=2959

[GenoMax]

You need to save the sequence data (use download or send to file) and then use a tool like JalView, Clustal Omega or MEGA to do the multiple sequence alignment. These tools would then allow you to highlight conserved residues.

[Tania]

thank you genomax so much