KEGG on sp. not in database

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7.0 years ago

Baylie_321 ▴ 30

Hiya,

I have DEG data on a species not in the KEGG databse which I would like to run KEGG enrichment analysis on. I was wondering what the best method would be please? I did a blast of the genome against two of the species I believe were the closest related and so have my own kind of database made, however most of the packages I have found require an ensembl/ref-seq etc.. ID and this gives no results when I try and run my lists from my data. Is there one that will do enrichment analysis on KEGG IDs, I haven't found one yet?

Best wishes,

Rebekah

RNA-Seq KEGG • 1.1k views

ADD COMMENT • link 7.0 years ago by Baylie_321 ▴ 30

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Okay, so I think I have a plan - I will download the fasta files from ensembl and blast my genome against this and then try these Is in KOBAS or DAVID. Does this sound correct? which fasta file should I be using to do this?

ADD REPLY • link 7.0 years ago by Baylie_321 ▴ 30

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