parsing fastq files in python
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Entering edit mode
7.0 years ago

I have multiple fastq files for different samples. Example : S_Rep1_R1.fastq S_Rep1_R2.fastq S_Rep2_R1.fastq S_Rep2_R2.fastq I want to choose all fastq files corresponding to say S_Rep1 and execute some functions. How can I write a python script which will choose all fastq files belonging to S_Rep1.

next-gen • 3.6k views
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Entering edit mode
7.0 years ago
st.ph.n ★ 2.7k
#!usr/bin/env python
import glob

for file in glob.glob('*S_Rep1*.fastq'):
        *do some stuff*
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1
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7.0 years ago
Hussain Ather ▴ 990

You could also use any standard file parser with python

#!usr/bin/env python
f = open("fastq_file.fastq", "r")

for i in f.readilnes():
    ....
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Entering edit mode
7.0 years ago

Here you go:

# importing os module
import os
# store the path of current directory in the variable 'cwd'
cwd = os.getcwd()
# loop over all files
for files in os.listdir(cwd):
    # check if file is fastq by extension
    if files.endswith(".fastq"):
        # check if file name contains 'S_Rep1'
        if 'S_Rep1' in files:
            # do something here
            print files

Keep calm and program ;)

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