Entering edit mode
7.0 years ago
phongphak.06
▴
20
Hi , I have tried to align WGS reads to viral reference genomes using Stampy software that the command I used is:
stampy.py -t 16 -g viral_ref -h viral_ref --bamkeepgoodreads -M SampleName.bwa.bam -o sample.bwa.stampy.sam
Note: This is a hybrid mode which was performed by using an alignment file from BWA as an input.
However, I got this error:
stampy: Error: Found reference ID (gi|167006425|ref|NC_010314.1|) in BAM which is not in genome reference while re-mapping with --bamkeepgoodreads. Check that the correct genome reference was selected.
So I have no idea how this was happened since I used the same reference when I aligned reads by using BWA and I already checked whether that ID is in the reference file, which is:
gi|167006425|ref|NC_010314.1| Abaca bunchy top virus DNA-N, complete genome
