Where to simulate RNA-seq data online

Question

[statfa]

Hi

I need to simulate some time course RNA-seq data. I wanted to use Polyester package in R. But I can't download those big Fasta files to run Polyester on my personal laptop. Is there any online site like Galaxy where I can simulate RNA-seq data?

Thanks a lot

[Hussain Ather]

If you can't download the big fast files on your personal laptop, you might consider using a virtual machine or ssh command to another system to run RNA-Seq.

[statfa]

Thank you so much. I will read about them.

[Kevin Blighe]

Did you take a look at this previous Biostars thread: Simulating Rna-Seq Reads

[statfa]

Thank you very much. I looked at it and the other page introduced there. They have introduced some tools which I will take a look at. But I have some questions before I read about those tools. I'm only limited to R and that is why I can't use some tools like fluxsimulator. If there is some online place where I can simulate data, that would be very helpful because then I don't need to download the data. Otherwise, do you know if there is a way to simulate data using a count matrix instead of Fasta and GTF files? This way I can obtain the count matrix from a real data set using galaxy and then simulate data using that count matrix. So I don't need to download those big files. How to simulate RNA-seq raw read counts matrix