when doing GSEA

Question

[mysskks]

I try to do GSEA. Therefore, I downloaded mRNA data files(GCT) from firebrowse. Then i did ranknormalization by using Gene pattern. After that, i opened GCT files by using EXCEL and figure out that tumor and normal tissue RNA data are mixing in. So can i exclude the normal tissue data ?? Then do GSEA?? or Include the tumor and normal tissue RNA data. Then do GSEA??

Thank for reading my question Pleas give comments please.

[Fabio Marroni]

It is really hard for me to understand your question. Please refer to the guide on how ask questions on biostars

[mysskks]

Thank you for your comments. I will read this and then question again.