samtools sort failed to merge tmp .bam file
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6.9 years ago
johnnytam100 ▴ 110

I tried to sort a .bam file with the following command

/data/tam/script/bwa.kit/samtools sort libTTNRgenomemaster_map_lotusgenome.bam > libTTNRgenomemaster_map_lotusgenome.sorted.bam

The following error, together with many ...bam.tmp.XXXX.bam files were generated (XXXX up to 1491)

[bam_sort_core] merging from 1492 files...
[E::hts_open_format] fail to open file 'libTTNRgenomemaster_map_lotusgenome.bam.tmp.1020.bam'

Related threads were read but not helpful.

Any idea how to solve this problem?

Thanks.

samtools bwa bam • 4.2k views
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Did you run out of space on the disk? How much memory do you have? You could try assigning more memory to sort with -m option. Post the output of ulimit -n.

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Hi, i am also having the same issue. THe output of ulimit -n is 1024 Thanks

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