Dear guys We ave sequenced the whole genome of a plant species (about 700Mb) using two technologies and the data output as bellow:
Illumina: PE 2*150, insert size 330-550bp, random sharing total reads : 606 037 434 total bases: 91 511 652 534 (90Gb)
IonTorrent : single read, average lenght 125bp, enzymatique sharing, total reads : 247 000 000 total bases : ~ 20 000 000 000 (20Gb)
My questions are : can we perform a denovo assembly? have you a methodological proposal to optimise the assembly (combining these two data sets ? Which software you suggest to use? Thank you i advance for you help! have a nice day to all.