Counting reads in CDS - but skipping few first/last codons
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6.9 years ago
PocketChange ▴ 10

I've been struggling with this for some time now, and finally decided to ask you guys for advice: I'm trying to analyze some ribosome profiling data, and would like to count reads (e.g. using HT-Seq) in cds, but at the same time I'd like to exclude several first and last codons (I think in general people skip first 15 and last 5 codons). I suppose I could potentially achieve this by modifying my gtf? Are there any tools you could recommend I use to get this done? Or do you have any other ideas/suggestions on how I start with this? Thank you for your help.

rna-seq htseq • 1.6k views
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I think bedtools can be used to change the gtf.

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More specifically: bedtools slop is able to increase the size of features. However, using negative integers for -l and -r shrinks features at the desired end.

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Thanks! So in this case proper way would be to extract CDS exons containing start codons, resize, and then merge back? Thanks again!

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