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6.9 years ago
MAPK
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I have 5 fastq(single-end) files from 5 replicates of wild-type samples. I want to see the read alignment percentage between each fastq file. Is there a way I can do this using bowtie or any other short read alignment alignment tools? Thank you for your help in advance.
The Log.final.out from STAR-Align might be useful.
What exactly does between mean? If you have already done the alignments then you can use something like Qualimap (or simply
samtools stats
) to generate statistics for alignments. If you usebbmap.sh
for alignments then it will generate detailed alignments stats for each run by default.