Size difference between Bam file and sort-bam file

Question

[mostafarafiepour]

Hi all,

My Bam Files Size is (33,831,754 kb), after sort Its size (24,478,862 kb). I want to know that it's right?

Thanks

Mostafa

[ATpoint]

Compression level is probably different. Check with this command on both files to see if you lost reads. Did you apply any filters in the sort command, please provide the full command.

samtools flagstat in.bam

[mostafarafiepour]

code I use:

samtools sort /home/m.rafiepour222/SRR3112430.bam SRR3112430-sort.bam

[ATpoint]

As I said, simply use flagstat to see if you lost any reads. If not, you'll be fine.

Answer 1

[Martombo]

that's expected, a sorted bam file can be further compressed than an unsorted one

[mostafarafiepour]

Ok, So my sorted bam file do not have a problem?