16S reads comparing

Question

[luyang1005]

Hi, community,

I have a question on the sequencing data comparison.

My reads are PAIR ENDED READS (150bp each end, merged are around 250bp) sequenced by Illumina 16S 515F-806R.

I want to analysis my reads together with EMP project data. However, I found their data are all 150bp SINGLE ENDED sequences.

May I know is there any way for me to pick the OTU together? And make them comparable?

Thanks in advance.

[sridhar56]

You should take a look at DADA2, will be useful for your comparison.

This paper will give you an idea on how the data can be comparable:

https://www.nature.com/articles/ismej2017119