1D^2: Oxford nanopre technology

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6.8 years ago

KVC_bioinfo ▴ 600

Hello all,

Recently, ONT introduced 1D^2 sequencing kit. I was wondering if someone has tried it. I want to know how different the fastq files look? Do we get 2 fastq files per sample (one per strand)? or it is one long file with improved accuracy?

TIA

ONT 1d2 • 1.8k views

ADD COMMENT • link 6.8 years ago by KVC_bioinfo ▴ 600

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I guess SEQanswers or ONT-community would be a better place for this question. You could also try Twitter!

ADD REPLY • link 6.8 years ago by Sej Modha 5.3k

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Thanks! I will try there.

ADD REPLY • link 6.8 years ago by KVC_bioinfo ▴ 600

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They've had 2D for a long time. By the time you got to FASTQs, it was indistinguishable from 1D (unless you specifically split template and complement strands). I assume the output would be similar for 1D^2.

ADD REPLY • link 6.8 years ago by igor 13k

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yes. I haven't seen ow the output from 2D looks like. Hence, I am confused. I have only seen how the output from 1D looks like.

ADD REPLY • link 6.8 years ago by KVC_bioinfo ▴ 600

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