Hi,
We are planning on doing two genetic screening experiments, one is done with Crispr plasmids, the second one is done via gene-trapping retrovirus. Even though the methods are different, the idea behind the analysis should be similar. in both data sets after infecting the cells with wither the lentivirus crispr library or the gene-trapping retrovirus, PCR is being used to enrich the population of the gRNA in the samples. The goal in both experiments is to identify gRNA (guide RNA) differentially expressed in one condition compared to a control sample. The interesting part in this kind of analysis is, that it is a much more specific method which gives back a small amount of genes to look at (a few hundreds genes in each library).
Beside the fact, that i am not strong on the biology behind these two procedures, I was wondering if someone can refer me to a paper / tutorial about how to analyze this kind of data.
I would appreciate recommendations on how to approach this kind of analysis.
thanks for any help. Assa
