Entering edit mode
6.7 years ago
mschmid
▴
180
I have PacBio and Oxford Nanopore reads mapped to a reference sequence. Since I have very long reads I had to use CRAM format.
Do you know a nice tool which calculates the aligment quality / mapping identity for every read from the CRAM alignment file?
Idealy it gives me also the the mapping length of the respective mapping.
How did you align the reads? Most tools able to parse BAM can also parse CRAM, so maybe
Picard CollectAlignmentSummaryMetrics
may do what you want?Partially. But I would like to have it per read. Not an average, to be able to plot a distribution of the alignment quality.