Entering edit mode
6.7 years ago
Sam
▴
150
Dear All
I have some problem with degradom files, the sequence length in raw reads file is 50 base , but in clean reads file (after removing the 3' adaptor) , about 20% of reads still have 50 bp length. is it due to adapator contamination ?
Thanks
This post looks helpful, some links don’t work, so I added 2 new links
StarScan: scanning small RNA targets in lncRNA, circRNA, mRNA and pseudo genes from degradome sequencing data
https://academic.oup.com/nar/article/43/W1/W480/2467966
StarScan: a web server for scanning small RNA targets from degradome sequencing data
The StarScan web server is available at http://mirlab.sysu.edu.cn/starscan/. But the site doesn;t answer.
This post below discusses a proper read length:
read length of sequence