Entering edit mode
6.8 years ago
nancy
▴
90
We would like to determine differentially expressed genes between two conditions. We are trying to use the Pertea et al pipeline (Hisat2+Stringtie+Ballgown). Following questions:
- should the fold change be computed using the fpkm measure or any other?
- If we use libadjust=FALSE, are the fold-changes simply derived arithmetically from fpkms or is there further normalization involved?
Thanks