ATAC-SEQ primers order

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Entering edit mode

6.7 years ago

clizama • 0

HI,

I'm starting a experiment with some cells from mice embryos. I want to be sure, my primers are correct before to do all the experiment.

I'm a little confused how to orders the primers for ATAC-seq: For each sample, I should be order:

Sample1:

Ad1_noMX: (Primer without index) Ad2.1_index: (Primer with index1)

Sample2: Ad1_noMX:(Primer without index) Ad2.2_index: (Primer with index2)

etc

In terms to order the primers if I follow the list from (Jason D Buenrostro paper https://media.nature.com/original/nature-assets/nmeth/journal/v10/n12/extref/nmeth.2688-S1.pdf):

I should be order:

Ad1_noMX: AATGATACGGCGACCACCGAGATCTACACTCGTCGGCAGCGTCAGATGTG

Basically the second primer I have to put the index sequence to order: index+sequence? Ad2.1_TAAGGCGA: TAAGGCGA-CAAGCAGAAGACGGCATACGAGATTCGCCTTAGTCTCGTGGGCTCGGAGATGT

Thanks

ChIP-Seq • 3.2k views

ADD COMMENT • link 6.7 years ago by clizama • 0

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Entering edit mode

You should post this over at SeqAnswers.com or biology stackexchange where predominantly experimental questions get quick answers.

Edit: I see that this is already cross-posted at SA.

ADD REPLY • link 6.7 years ago by GenoMax 147k

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