How to analyze the expression of a single gene in microarray data?

Question

[Leite]

Hello everyone

I would like to know if there is a way to analyze the variation of the expression in different samples of microarray, without necessarily doing analysis of DEGs.

Best regards,

Leite

[h.mon]

The title and the question don't exactly match - your title says you are interested on a single gene, but the body of the question don't.

Besides, what do you mean by "analyze the variation of the expression in different samples of microarray, without necessarily doing analysis of DEGs"? One can, for example, perform pathway enrichment analysis without performing differential gene expression. Is this the kind of answer you are looking for?

[Leite]

For example, I am interested in knowing the expression of ADM (adrenomedullin) in a sample of patients with infection.

I am then downloading several GEO databases and doing the DEGs analysis and seeing if I find ADM gene in that data, but this is taking a lot of time and is not generating interesting results. I wonder if there is a way to do this, look up the expression of a particular gene in a given sample without having to reanalysis the database.

[Leite]

Something like Search for Gene Expression at GEO Profiles But something more robust that I can use in a paper.

Answer 1

[Hussain Ather]

Differential gene expression would be the best way to go. Otherwise there are methods using dupRadar, featureCounts, HTSeq for measuring values that may be of interest to you.