I have few Differentially expressed miRNAs out of edgeR ?

Question

[Björn]

I have found Differentially expressed miRNAs out of edgeR. What are next steps for pathway analysis ? Any literature would be appreciated. I used Targetscan to find genes that are associated with individual miRNAs. But I believe there are many other steps to perform before I can finally prepare result to publish.

Answer 1

[Kevin Blighe]

Hi, I have used miRNAtap a few times in the past, including recently. It finds target genes of your statistically significant micro RNAs. You can then feed these target genes into enrichment programs like topGO and KEGGprofile. See my answer here: A: Identifying miRNA, RNA-binding sites in UTRs

Kevin