Is TCGA PRAD RNA-seq data strand specific?
2
2
Entering edit mode
8.6 years ago
halffedelf ▴ 40

I have been trying to find if TCGA PRAD (prostate cancer) RNA-seq data strand specific or not. I can not find any relevant info. I did find that it is Illumina protocol, but nothing more. Does anyone if they are strand specific? Do you have the right link? Thanks

RNA-Seq TCGA PRAD strand • 4.4k views
ADD COMMENT
1
Entering edit mode

at the top of my head I don't know, however, there is a similar questions here on Biostar if you can find out the library preparation method/kit that might help.

ADD REPLY
3
Entering edit mode
6.6 years ago
neethav ▴ 40

All TCGA samples are non-stranded.

ADD COMMENT
1
Entering edit mode
5.8 years ago
Zhenyu Zhang ★ 1.2k

The short answer is no.

The specific RNA-Seq library preparation kit TCGA used actually "could" support stranded library; however, it was used in a manner that libraries are actually strandless.

ADD COMMENT

Login before adding your answer.

Traffic: 2443 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6