No output nr file after running makedb
0
0
Entering edit mode
6.6 years ago
Chvatil ▴ 130

I'm actually trying to creat a database for diamond with this script:

#PBS -S /bin/bash
#PBS -l nodes=1:ppn=2
#PBS -e /pandata/me/blast_database/makedb.error
#PBS -o /pandata/me/blast_database/makedb.out
#PBS -N makedb2
#PBS -q q1hour
hostname
uname -a

echo debut:
date  #debut 

diamond=/panhome/me/miniconda3/bin/diamond 

nr_faa=/pandata/me/blast_database/nr.faa

$diamond makedb --in $nr_faa -d nr2

So I ran it an it actually worked without error (makedb.error file is empty) but I cannot find the nr2 outfile nowhere and I do not know why? It ran on a cluster. Thank for your help.

blast diamond makedb • 3.1k views
ADD COMMENT
0
Entering edit mode

As I recall DIAMOND needs a lot of memory. You don't seem to have specified any additional beyond your default allocation. I will need to look this up but I may have used 80G or so last time I made the indexes.

ADD REPLY
0
Entering edit mode

With 30 GB is not enought? because I already ran it with less that that, here I do not understand why it does not work. I'll try with :bigmem,mem=30gb and let you know

ADD REPLY
0
Entering edit mode

Hi still get the same probleme, everything seems to work but I cannot see the file, if. it was a memory probleme I should get an error message no?

ADD REPLY
0
Entering edit mode

Do you have write permissions on the directory where you are running this job from? Since you are not specifying an output directory/path, DIAMOND will try to write nr2 to the current directory.

ADD REPLY
0
Entering edit mode

Well, if I do $diamond makedb --in $nr_faa -d directory/path/nr2 will it work? or it accepts aonly a name?

ADD REPLY
0
Entering edit mode

Try to see if it already present somewhere else on your system by using @h.mon's suggestion.

Otherwise try the command above. That file should be 60+G so you are not going to miss it.

ADD REPLY
0
Entering edit mode

What is the output of stdout and stderr? Did you try to find the diamond db:

find ~ -name "nr2.dmnd"
ADD REPLY
0
Entering edit mode

This command does not work, when I use it with a file name that exists, nothing appends (I'm in a virtual env - server)

ADD REPLY
0
Entering edit mode

nothing appends

Do you mean "nothing happens"? That command is doing a search with that file name.

ADD REPLY
0
Entering edit mode

Toto26 : You may still be working on/through this question.

In case the question has been solved then indicate which of the following comments was useful (or if you found an independent solution then post that as an answer). I can move that comment to an answer so it can be accepted.

ADD REPLY

Login before adding your answer.

Traffic: 1819 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6