error in using LOLA enrichment analysis in RnBeads

Question

[rahel14350]

Dear all, I am doing Diff. meth. analysis for RRBS data using RnBeads. I am interested to do enrichment analysis from Lola package here but I have this error:

>Error in rnb.getOption(differential.enrichment.lola = TRUE) : 
 >unused argument (differential.enrichment.lola = TRUE)

Can you please help me? Kind Regards, Rahel

[WouterDeCoster]

Please elaborate by adding the code you used when you obtained this error.

[rahel14350]

I want to start RnBeads full pipeline and in the beginning with setting rnb.options, I have this error. Here is my options:

>rnb.options(
    exploratory.intersample           = FALSE,
    exploratory.region.profiles       = NULL, 
    differential.enrichment.go=T,
    differential.enrichment.lola=T,
    differential.enrichment.lola.dbs = c("${LOLACore}"),
    differential.comparison.columns   = c("sample_Group"),
    differential.report.sites         = TRUE
)

I am using RnBeads.1.12.0 version.

[WouterDeCoster]

I added code markup to your post for increased readability. You can do this by selecting the text and clicking the 101010 button. When you compose or edit a post that button is in your toolbar, see image below:

[WouterDeCoster]

I am using RnBeads.1.12.0 version.

Then my first suggestion is to update to the latest version.

[rahel14350]

Many thanks for your prompt reply. The last version is 1.12.1 and I have the same Error there as well. I also have LOLA installed and included that one too (require(LOLA)) but still the same error is happening.

[WouterDeCoster]

Oh I was confused for a second, you indeed have the latest version. My bad.

Can you post the output of sessionInfo()?

[rahel14350]

Here is:

    > sessionInfo()
R version 3.4.4 (2018-03-15)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 16.04.4 LTS

Matrix products: default
BLAS: /usr/lib/libblas/libblas.so.3.6.0
LAPACK: /usr/lib/lapack/liblapack.so.3.6.0

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
 [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8    
 [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
 [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
 [9] LC_ADDRESS=C               LC_TELEPHONE=C            
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       

attached base packages:
 [1] grid      stats4    parallel  stats     graphics  grDevices utils    
 [8] datasets  methods   base     

other attached packages:
 [1] LOLA_1.7.1                             
 [2] RnBeads_1.12.1                         
 [3] plyr_1.8.4                             
 [4] methylumi_2.22.0                       
 [5] minfi_1.22.1                           
 [6] bumphunter_1.16.0                      
 [7] locfit_1.5-9.1                         
 [8] iterators_1.0.9                        
 [9] foreach_1.4.4                          
[10] Biostrings_2.44.2                      
[11] XVector_0.16.0                         
[12] SummarizedExperiment_1.6.5             
[13] DelayedArray_0.2.7                     
[14] FDb.InfiniumMethylation.hg19_2.2.0     
[15] org.Hs.eg.db_3.4.1                     
[16] TxDb.Hsapiens.UCSC.hg19.knownGene_3.2.2
[17] GenomicFeatures_1.28.5                 
[18] AnnotationDbi_1.38.2                   
[19] reshape2_1.4.3                         
[20] scales_0.5.0.9000                      
[21] Biobase_2.36.2                         
[22] illuminaio_0.18.0                      
[23] matrixStats_0.53.1                     
[24] limma_3.32.10                          
[25] gridExtra_2.3                          
[26] gplots_3.0.1                           
[27] ggplot2_2.2.1.9000                     
[28] fields_9.6                             
[29] maps_3.3.0                             
[30] spam_2.1-4                             
[31] dotCall64_0.9-5.2                      
[32] ff_2.2-13                              
[33] bit_1.1-12                             
[34] cluster_2.0.7-1                        
[35] MASS_7.3-50                            
[36] GenomicRanges_1.28.6                   
[37] GenomeInfoDb_1.12.3                    
[38] IRanges_2.10.5                         
[39] S4Vectors_0.14.7                       
[40] BiocGenerics_0.22.1                    

loaded via a namespace (and not attached):
 [1] nlme_3.1-137             bitops_1.0-6             bit64_0.9-7             
 [4] httr_1.3.1               RColorBrewer_1.1-2       tools_3.4.4             
 [7] doRNG_1.6.6              nor1mix_1.2-3            R6_2.2.2                
[10] KernSmooth_2.23-15       DBI_1.0.0                lazyeval_0.2.1          
[13] colorspace_1.3-2         withr_2.1.2              base64_2.0              
[16] preprocessCore_1.38.1    compiler_3.4.4           pkgmaker_0.22           
[19] rtracklayer_1.36.6       caTools_1.17.1           quadprog_1.5-5          
[22] genefilter_1.58.1        stringr_1.3.1            digest_0.6.15           
[25] Rsamtools_1.28.0         siggenes_1.50.0          GEOquery_2.42.0         
[28] pkgconfig_2.0.1          rlang_0.2.0.9000         RSQLite_2.1.1           
[31] mclust_5.4               BiocParallel_1.10.1      gtools_3.5.0            
[34] RCurl_1.95-4.10          magrittr_1.5             GenomeInfoDbData_0.99.0 
[37] Matrix_1.2-14            Rcpp_0.12.16             munsell_0.4.3           
[40] stringi_1.2.2            zlibbioc_1.22.0          blob_1.1.1              
[43] gdata_2.18.0             lattice_0.20-35          splines_3.4.4           
[46] annotate_1.54.0          multtest_2.32.0          beanplot_1.2            
[49] pillar_1.2.2             rngtools_1.2.4           codetools_0.2-15        
[52] biomaRt_2.32.1           XML_3.98-1.11            data.table_1.11.2       
[55] gtable_0.2.0             openssl_1.0.1            reshape_0.8.7           
[58] xtable_1.8-2             survival_2.42-3          tibble_1.4.2            
[61] GenomicAlignments_1.12.2 registry_0.5             memoise_1.1.0

[WouterDeCoster]

I don't see lola here listed.

[rahel14350]

sorry, edited now! even now I still have the error!

[WouterDeCoster]

My apologies for being lazy, I'll see if I can reproduce your issue.

[WouterDeCoster]

I was unable to reproduce your issue with LOLA_1.8.0 and RnBeads_1.10.8 (command works fine).

[rahel14350]

I changed the version and unfortunately, I still have the same error!