Entering edit mode
9.1 years ago
chenzizhen01
▴
20
I'm dealing with RNApolyII chipseq data using MACS2.
RNApolyII chipseq data contains two kinds of peaks:narrow peaks at TSS region and broad peaks across genebody.
So, how to set options of MACS2?
DO I have to run MACS2 twice? First, run macs2 callpeak
without --broad
in order to find narrow peaks at TSS. Then run macs2
with --broad
to find broad peaks?
If I do that, should I run MACS2 bdgdiff
separately if I want to find difference between two different samples?
What did you do in the end?