Spike-in RNA in single cell experiment

Question

[nicolas.hipp]

Hello everyone,

I look to setup a single cell-RNAseq experiment with the C1 fluidigm platform. In the protocol, the advice to use Spike-in RNA for normalization (Ambion AM1780). So I look on the website of thermofisher, but I cannot find any sequences of these Spike-in. I made some research, and peoples are used to modify the index file to align these control, but without the sequence I don't know how to do that.

Does anybody used these spike-in for experiments, and have a solution to implement the index file to align reads?

Thanks a lot,

nicolas

Ps: I sent an email to the support, currently waiting for an answer.

Answer 1

[nicolas.hipp]

Support sent me the sequences. sorry for this useless post ;)

[haoxuanl]

would you sent me a copy? I'm looking for the same thing. my email is [e-mail] Thanks!

[djc22591]

Did the poster send you them? I'm looking for them also. I will try the companies support however if you have them could you send them to [e-mail]? thanks.