How to remove primers from a sequencing run with cutadapt
0
0
Entering edit mode
6.5 years ago
Javad ▴ 150

Dear all,

I would like to use cutadapt to trim the primers from my sequencing run. I was just wondering when I give the sequence of my primer to cutadapt, do I also have to give the complementary sequence as well?

for example let's say my primer is: AATC

Then is this command "cutadapt -g AATC input.fa output.fa" sufficient to remove the primer sequence or I also have to run the following command:

cutadapt -g TTAG input.fa output.fa

Thanks in advance

RNA-Seq • 2.9k views
ADD COMMENT
0
Entering edit mode

from the manual:

Replace "ADAPTER" with the actual sequence of your 3' adapter. IUPAC wildcard characters are supported. The reverse complement is not automatically searched.

ADD REPLY

Login before adding your answer.

Traffic: 1625 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6