DADA2 vs DEBLUR
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6.9 years ago

Hi all!

I'm a PhD student working on Microbiome and Mycobiome in skin dog! I'm learning how to use QIIME2 for sequences analysis.

I saw that in QIIME2 you can use or DADA2 or Deblur for the analysis, and my question is: what do you think about them?? which one is better?

Thank you very much, any help will be very appreciated!

Best regards

Sara

sequence sequencing next-gen • 6.0k views
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Thank you for your reply!

I obtained my sequencing data from IonTorrent and I'm sequencing the 16S gene. As I'm not an expert of DADA2 and Deblur, I asked opinions about them to understand if both were fine for these analysis!!

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Americans are really preferring DADA2 for no reasons. Last month I attended this course QIIME2 with Antonio and Tomasz (who developed qiime) and they were all saying its your choice to choose. In any case it is the interpretation at the end. All the test sessions were also based on DADA2 and so I also used it for my dataset. The same recommendation I would give you as well!

best

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arsilan324 and sara.dandreano : Please use ADD COMMENT/ADD REPLY when responding to existing posts to keep threads logically organized.

Submit Answer should only be used for new answers to original question.

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arsilan324 Really thank you for your reply!!!!!

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You are welcome! If you have any questions regarding workflow, you can reach me ..

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Please keep all discussion on the forum so it benefits everyone.

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Ok so Deblur authors are not recommending Deblur in their workshops?

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6.9 years ago
arsilan324 ▴ 90

This is really personal choice and also based on your dataset. If your sequencing data is Illumina MiSeq/HiSeq I would suggesting using DADA2

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