gene name from bed file using R language of cell lines
1
0
Entering edit mode
6.5 years ago

what packages of r can be used to analyse the bed files. i want to align the bed file to genome data to get the gen name of the bed file. the syntax mention below has suggested by many but its not working over

annotatePeaks.pl  <peak/BED file> <genome>   > <output file>
R RNA-Seq • 2.0k views
ADD COMMENT
0
Entering edit mode

C'mon man, with that kind of sloppy and undetailed question, you'll not get any elaborate response. Provide some details what data you have, what you've tried and what the final goal is.

ADD REPLY
0
Entering edit mode

Try again, but this time, be polite.

ADD REPLY
0
Entering edit mode

this was my first time i will not repeat next time

ADD REPLY
0
Entering edit mode

That comment was directed at ATpoint, not you. ATpoint is right, you have not included enough details in your question, but their way of saying it was incredibly rude.

ADD REPLY
0
Entering edit mode

You are not wrong, but you could have phrased it much better. It may not be obvious for new users which information is important. Also language barrier problems may make questions unclear. The last thing we want is that this forum becomes a 'hostile' environment which scares away those users which need help the most.

Besides reactions like this (which I would call "too direct") we absolutely value your contribution to biostars. Thank you for being an active member!

ADD REPLY
0
Entering edit mode

this was my first time i will not repeat next time

ADD REPLY
0
Entering edit mode

this was my first time i will not repeat next time

ADD REPLY
2
Entering edit mode
6.5 years ago
steve ★ 3.5k

the syntax mention below has suggested by many but its not working over

annotatePeaks.pl <peak bed="" file=""> <genome> > <output file="">

That is not R, that is part of a separate program called HOMER.

There are several example methods of annotating .bed files (e.g. getting the gene names for coordinates in the file) listed here:

https://github.com/stevekm/annotate-peaks

Including methods using R packages.

ADD COMMENT
0
Entering edit mode

Thank you I will check it out

ADD REPLY

Login before adding your answer.

Traffic: 1657 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6