Question

RNA seq saturation analysis

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6.5 years ago

shahbaz.ahmed ▴ 40

Could someone please explain what saturation analysis is and how does it help with RNA sequencing data analysis?

rna-seq • 5.4k views

ADD COMMENT • link updated 6.5 years ago by h.mon 35k • written 6.5 years ago by shahbaz.ahmed ▴ 40

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Did you check this thread? Tools For Saturation Analysis Of Rna-Seq Experiments

ADD REPLY • link 6.5 years ago by venu 7.1k

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Thanks for the reply. I did but it does not quite describe why is this analysis used at first place or what kind of information it generates. I'm a beginner.

ADD REPLY • link 6.5 years ago by shahbaz.ahmed ▴ 40

score 1 · Answer 1 · 2018-05-30

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6.5 years ago

h.mon 35k

In a nutshell, to see if you sequenced enough. It is late night, so I will defer the explanation to these links here:

https://cofactorgenomics.com/1-graph-will-give-you-new-perspective-sequencing-experiment/

https://cofactorgenomics.com/stop-sequencing/

ADD COMMENT • link 6.5 years ago by h.mon 35k

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Thanks, the link was helpful

ADD REPLY • link 6.5 years ago by shahbaz.ahmed ▴ 40

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Are there are any scripts/tools that perform this analysis easily? I've looked and can't find anything off the shelf.

ADD REPLY • link 6.4 years ago by achamess ▴ 90