Entering edit mode
6.5 years ago
inayat45shaikh
▴
40
I am trying to run micropred for feature assessment from microRNA sequences, but getting following error
[inayat@gibhpc1 progs]$ perl microPred.pl seq.fasta
-microPred started...
-calculating features-------------
-folding original sequences...
-calculating miPred sequential and structural features...
perl: symbol lookup error: ./miPred/ViennaRNA-1.6.4/Perl/blib/arch/auto/RNA/RNA.so: undefined symbol: Perl_Gthr_key_ptr
-calculating z-features...(this might take some time as 1,000 random sequences are generated for each original sequence)
perl: symbol lookup error: ./miPred/ViennaRNA-1.6.4/Perl/blib/arch/auto/RNA/RNA.so: undefined symbol: Perl_Gthr_key_ptr
-calculating MFEI1, MFEI2, MFEI3, MFEI4.....
-calculating basepair-related features...
-calculating RNAfold-related features...
-calculating mfold-related features...
---------------------------
-filtering all features...
java.io.FileNotFoundException: ../data/seq.fasta.zdata (No such file or directory)
at java.io.FileInputStream.open0(Native Method)
at java.io.FileInputStream.open(FileInputStream.java:195)
at java.io.FileInputStream.<init>(FileInputStream.java:138)
at java.io.FileInputStream.<init>(FileInputStream.java:93)
at filter_all.main(filter_all.java:16)
-formating features...
java.io.FileNotFoundException: ../data/selected.seq.fasta.-21.features (No such file or directory)
at java.io.FileInputStream.open0(Native Method)
at java.io.FileInputStream.open(FileInputStream.java:195)
at java.io.FileInputStream.<init>(FileInputStream.java:138)
at java.io.FileInputStream.<init>(FileInputStream.java:93)
at format1.main(format1.java:12)
-scaling features into [-1,+1]...
can't open file ../SVM/seq.fasta.formatted
-predicting...
---------------------------------
-Results...
-Prediction results are written to the file /data/seq.fasta.predict
(+1 indicates a pre-miRNA candidate, -1 indicates a negative candidate)
-All features extracted are written to the file /data/all.seq.fasta.-48.features
-Best 21 features used for the prediction are written to the file /data/selected.seq.fasta.-21.features
-microPred completed successfully...
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