difficulty in executing micropred
0
1
Entering edit mode
6.5 years ago

I am trying to run micropred for feature assessment from microRNA sequences, but getting following error

[inayat@gibhpc1 progs]$ perl microPred.pl seq.fasta
-microPred started...
-calculating features-------------
    -folding original sequences...
    -calculating miPred sequential and structural features...
perl: symbol lookup error: ./miPred/ViennaRNA-1.6.4/Perl/blib/arch/auto/RNA/RNA.so: undefined symbol: Perl_Gthr_key_ptr
    -calculating z-features...(this might take some time as 1,000 random sequences are generated for each original sequence)
perl: symbol lookup error: ./miPred/ViennaRNA-1.6.4/Perl/blib/arch/auto/RNA/RNA.so: undefined symbol: Perl_Gthr_key_ptr
    -calculating MFEI1, MFEI2, MFEI3, MFEI4..... 
    -calculating basepair-related features...
    -calculating RNAfold-related features...
    -calculating mfold-related features...
---------------------------
    -filtering all features...
java.io.FileNotFoundException: ../data/seq.fasta.zdata (No such file or directory)
    at java.io.FileInputStream.open0(Native Method)
    at java.io.FileInputStream.open(FileInputStream.java:195)
    at java.io.FileInputStream.<init>(FileInputStream.java:138)
    at java.io.FileInputStream.<init>(FileInputStream.java:93)
    at filter_all.main(filter_all.java:16)
    -formating features...
java.io.FileNotFoundException: ../data/selected.seq.fasta.-21.features (No such file or directory)
    at java.io.FileInputStream.open0(Native Method)
    at java.io.FileInputStream.open(FileInputStream.java:195)
    at java.io.FileInputStream.<init>(FileInputStream.java:138)
    at java.io.FileInputStream.<init>(FileInputStream.java:93)
    at format1.main(format1.java:12)
    -scaling features into [-1,+1]...
can't open file ../SVM/seq.fasta.formatted
    -predicting...
---------------------------------
-Results...
    -Prediction results are written to the file /data/seq.fasta.predict 
      (+1 indicates a pre-miRNA candidate, -1 indicates a negative candidate)
    -All features extracted are written to the file /data/all.seq.fasta.-48.features
    -Best 21 features used for the prediction are written to the file /data/selected.seq.fasta.-21.features
-microPred completed successfully...
----------------------------------
RNA-Seq • 1.1k views
ADD COMMENT

Login before adding your answer.

Traffic: 2977 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6