How to perform eQTL study if i have small sample size?

Question

[akilabioinfo]

Hi All,

I would like to study the variant effect in gene expression (eQTL) Currently, I am having 4 RNA seq sample for the disease condition. I would like to do eQTL study based on this sample. Is it feasible to do? relse can i download the microarray data from GEO and proceed further?. I am a beginner for an eQTL study. Please advice me.

Answer 1

[maxime.policarpo]

I would say this is impossible, usually QTL studies use hundreds of sequenced individuals. That permit a good heterogeneity of their genome due to random recombination and a good difference of segregation between molecular markers and phenotype.

[cao510927]

Hi, maxime! what's about 15 samples? is it appropriate to do QTL analysis? Or, is there any way to filter the MatrixEQTL results?