Polyester RNA-seq simulation

Question

[k.kathirvel93]

Hi EveryOne,

I am trying Polyester tool to simulate RNA-seq reads. i got a R code, but that will produce reads from chr22, but i want to generate reads from all chromosomes. I am very new to R. Can i get code or suggestions. Thanks in advance.

[GenoMax]

Without actual code you are using it is difficult for people to see what is going on. Please edit the original post and add your code/output samples.

[k.kathirvel93]

Here is the github manual which i have used. Thank you

Answer 1

[GenoMax]

The manual is only using sequence for chr22 as an example.

fasta_file = system.file('extdata', 'chr22.fa', package='polyester')

You will need to replace chr22.fa with a genome.fa, if you need the full genome.