Entering edit mode
6.4 years ago
StudentBio
•
0
hello community, please when I try to visualize the mapping results in IGV i get this error,please any suggestion
File: /home/vshare/mapping/reads_aligned.sorted.bam does not contain any sequence names which match the current genome. File: NW_008246507.1, NW_008246508.1, NW_008246509.1, NW_008246510.1, ... Genome:
looks like your bam file does not contain the same sequence IDs as the fasta genome file (or vice cersa)
please how can i correct it
Did you create a custom genome for your data in IGV?
no what that mean a custom data
Accession numbers in your original post refer to
Phoenix dactylifera. Is that the genome you are working with? A custom genome can be created using the fasta sequence that you used for aligning your data against and (optionally) an annotation file (in GTF format), if available.You can see the directions on how to create a custom genome by visiting the link I posted above.
When i followed the link i got the following message:
Access denied You are not authorized to access this page.
See: https://software.broadinstitute.org/software/igv/LoadGenome
Check
loading other genomessection. I will update the link above.