Phyloseq R error: Error in if (xi > xj) 1L else -1L : missing value where TRUE/FALSE needed

Question

[arsilan324]

Hi all,

I am trying to use phyloseq package in R for plotting 16S amplicon sequencing results. But I get this error after running the command.

> plot_bar(physeq2, fill = "Annotaton")   
> Error in if (xi > xj) 1L else -1L : missing value where TRUE/FALSE needed

I am using the data whose subset looks like this,

SR2_S84 SR3_S85 SR4_S89 SR5_S90 SR6_S91
0   0   0   0   0
0   0.000666183 0   0   0
0   0   0   0   0
0.007258343 0.005995643 0.001528514 0.002698655 0.002695369

Can you please comment what mistake I am doing? Thanks

[cpad0112]

Could you please post example data?

[arsilan324]

A subset looks like this

SR2_S84 SR3_S85 SR4_S89 SR5_S90 SR6_S91
0   0   0   0   0
0   0.000666183 0   0   0
0   0   0   0   0
0.007258343 0.005995643 0.001528514 0.002698655 0.002695369

When I use the actual numbers, it works; but when i use the percent data (re-standardized), it gives error.

Thanks

[Ram]

Please edit your question and add this detail in.