filtering a z score file

Question

[kanwarjag]

I analyzed Sc-RNAseq data set and has a z value score for each cell and probable cell type. The file looks like :

 type                               Cell1               Cell2                  Cell3                  Cell4                 Cell5
Endoderm_       3.496226196 1.869410134 1.526973971 0.738600081 0.773102146
Progenitor          3.429614031 1.655018262 1.281466081 0.714519041 0.805770879
Activin_d5          3.601420798 2.430262477 1.748683431 1.78143451  1.600960998

So I have 1200 columns and around 100 rows. I want to assign each col (cell) with unique most probable type. It may be a very straight forward task however I could not figure out how I can assign each cell to most likely type.

Any one has worked with Zscore filtration please advice. I tried filtration etc with a cutoff of Z score but could not reach at a solution.

[ATpoint]

It may be a very straight forward task

It is IMHO rather one of the most delicate tasks in scRNA-seq, so the question is why you do not use an established tool for this, rather than a homemade solution? Do you have a reason not to use existing tools?

Answer 1

[kanwarjag]

Unfortunately it is an out put of a standard tool Altanalyze and I could not get any reply from authors. Do you have any suggestion of a standard tool that can help me in this?

Thanks

Answer 2

[rob.costa1234]

I am also looking how to filter z score file as stated above. Any one here in the forum, has experience working on such files.