Entering edit mode
6.4 years ago
juara
▴
40
Hello
I have a rather naive question. I ran GATK4 mutect2 on my samples with the following flags:
--contamination-fraction-to-filter 0.0 --max-reads-per-alignment-start 0
The former is to disable downsampling process. So, theoretically I should be able to have equal values in t_depth column (total count of reads per variant) compared to total number of reads aligned on that locus. But they do not match at all.
Any comment would be appreciated. I have no idea what I am missing here.
Thanks