RNA seq saturation analysis

Question

[shahbaz.ahmed]

Could someone please explain what saturation analysis is and how does it help with RNA sequencing data analysis?

[venu]

Did you check this thread? Tools For Saturation Analysis Of Rna-Seq Experiments

[shahbaz.ahmed]

Thanks for the reply. I did but it does not quite describe why is this analysis used at first place or what kind of information it generates. I'm a beginner.

Answer 1

[h.mon]

In a nutshell, to see if you sequenced enough. It is late night, so I will defer the explanation to these links here:

https://cofactorgenomics.com/1-graph-will-give-you-new-perspective-sequencing-experiment/

https://cofactorgenomics.com/stop-sequencing/

[shahbaz.ahmed]

Thanks, the link was helpful

[achamess]

Are there are any scripts/tools that perform this analysis easily? I've looked and can't find anything off the shelf.