Split-Seq full length

Question

[Sarah]

Hi,

Does the Split-Seq protocol yield full length or 3' sequencing?

From the paper it looks like it is 3' but they also use random hexamer priming.

Thank you!

[Joe]

I'll happily make way for people if they have answers but I suspect this question might be better over on SeqAnswers instead.

Answer 1

[h.mon]

According to the review A practical guide to single-cell RNA-sequencing for biomedical research and clinical applications, it is 3'-end sequencing.