obtaining a concatenamer of sequences

Question

[annalisa79]

Ther all I was wondering if anyone could help me in obtaining a concatenamer of sequences in the way showed below. I have several multifasta files relative a genes sequences (ABC, GHJ…) in different organisms (>182680572 , >749299147…)

Gene ABC

>182680572
ATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTTCCACCAGAAGATGTTCATACTTGGTTGAGACCTTTACAAGCTGACCAACGCGGTGACAGTGTCATCCTTTACGCACCCAATACCTTTATCATTGAACTAGTAGAAGAGCGATA
>749299147
ATGACAACATTGATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTTCCACCAGAAGATGTTCATACTTGGTTGAGACCTTTACAAGCTGACCAACGCGGTGACAGTGTCATCCTTTACGCACCCAATACCTTTATCATTGAACTAGTAGAAGAGCGATACTTAGGGCGTCTTCGAGAATTGTTATCCTATTTTTCAGGAATACGTGAAGTAGTCCTTGCAATTGGCA
>584117620
ATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTTCCACCAGAAGATGTTCATACTTGGTTGAGACCTTTACAAGCTGACCAACGCGGTGACAGTGTCATCCTTTACGCACCCAATACCTTTATCATTGAACTAGTAGAAGAGCGATACTTAGGGCGTCTTCGAGAATTGTTATCCTATTTTTCAGGAATACGTGAAGTAGTCCTTGCAATTGGCTCACGACCTAA
>985743106
ATGACAACATTGATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTCCCGCCAGAAGATGTTCATACTTGGTTAAGACCTTTACAAGCCGACCAACGTGGTGACAGTGTCGTCCTTTACGCACCGAATCCCTTTATCATTGAACTAGTAGAAGAGCGATACTTAGGACGTCTTCGGGAATTGTTATCCTATTTTTCAGGAATACGTGAAGTAGTCCTTGCAATTGG

GENE GHJ

>182680572
ATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTTCCACCAGAAGATGTTCATACTTGGTTGAGACCTTTACAAGCTGACCAACGCGGTGACAGTGTCATCCTTTACGCACCCAATACCTTTATCATTGAACTAGTAGAAGAGCGATA
>749299147
ATGACAACATTGATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTTCCACCAGAAGATGTTCATACTTGGTTGAGACCTTTACAAGCTGACCAACGCGGTGACAGTGTCATCCTTTACGCACCCAATACCTTTATCATTGAACTAGTAGAAGAGCGATACTTAGGGCGTCTTCGAGAATTGTTATCCTATTTTTCAGGAATACGTGAAGTAGTCCTTGCAATTGGCA
>584117620
ATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTTCCACCAGAAGATGTTCATACTTGGTTGAGACCTTTACAAGCTGACCAACGCGGTGACAGTGTCATCCTTTACGCACCCAATACCTTTATCATTGAACTAGTAGAAGAGCGATACTTAGGGCGTCTTCGAGAATTGTTATCCTATTTTTCAGGAATACGTGAAGTAGTCCTTGCAATTGGCTCACGACCTAA
>985743106
ATGACAACATTGATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTCCCGCCAGAAGATGTTCATACTTGGTTAAGACCTTTACAAGCCGACCAACGTGGTGACAGTGTCGTCCTTTACGCACCGAATCCCTTTATCATTGAACTAGTAGAAGAGCGATACTTAGGACGTCTTCGGGAATTGTTATCCTATTTTTCAGGAATACGTGAAGTAGTCCTTGCAATTGG

Then I want to obtain for each organism a concatened sequence of the genes in the same order for each organisms

>182680572
ATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTTCCACCAGAAGATGTTCATACTTGGTTGAGACCTTTACAAGCTGACCAACGCGGTGACAGTGTCATCCTTTACGCACCCAATACCTTTATCATTGAACTAGTAGAAGAGCGATAATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTTCCACCAGAAGATGTTCATACTTGGTTGAGACCTTTACAAGCTGACCAACGCGGTGACAGTGTCATCCTTTACGCACCCAATACCTTTATCATTGAACTAGTAGAAGAGCGATA
>749299147
ATGACAACATTGATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTTCCACCAGAAGATGTTCATACTTGGTTGAGACCTTTACAAGCTGACCAACGCGGTGACAGTGTCATCCTTTACGCACCCAATACCTTTATCATTGAACTAGTAGAAGAGCGATACTTAGGGCGTCTTCGAGAATTGTTATCCTATTTTTCAGGAATACGTGAAGTAGTCCTTGCAATTGGCAATGACAACATTGATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTTCCACCAGAAGATGTTCATACTTGGTTGAGACCTTTACAAGCTGACCAACGCGGTGACAGTGTCATCCTTTACGCACCCAATACCTTTATCATTGAACTAGTAGAAGAGCGATACTTAGGGCGTCTTCGAGAATTGTTATCCTATTTTTCAGGAATACGTGAAGTAGTCCTTGCAATTGGCA
>584117620
ATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTTCCACCAGAAGATGTTCATACTTGGTTGAGACCTTTACAAGCTGACCAACGCGGTGACAGTGTCATCCTTTACGCACCCAATACCTTTATCATTGAACTAGTAGAAGAGCGATACTTAGGGCGTCTTCGAGAATTGTTATCCTATTTTTCAGGAATACGTGAAGTAGTCCTTGCAATTGGCTCACGACCTAA ATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTTCCACCAGAAGATGTTCATACTTGGTTGAGACCTTTACAAGCTGACCAACGCGGTGACAGTGTCATCCTTTACGCACCCAATACCTTTATCATTGAACTAGTAGAAGAGCGATACTTAGGGCGTCTTCGAGAATTGTTATCCTATTTTTCAGGAATACGTGAAGTAGTCCTTGCAATTGGCTCACGACCTAA
>985743106
ATGACAACATTGATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTCCCGCCAGAAGATGTTCATACTTGGTTAAGACCTTTACAAGCCGACCAACGTGGTGACAGTGTCGTCCTTTACGCACCGAATCCCTTTATCATTGAACTAGTAGAAGAGCGATACTTAGGACGTCTTCGGGAATTGTTATCCTATTTTTCAGGAATACGTGAAGTAGTCCTTGCAATTGGATGACAACATTGATGGAATCTTGGTCCCGTTGCCTGGAACGTCTTGAAACTGAATTCCCGCCAGAAGATGTTCATACTTGGTTAAGACCTTTACAAGCCGACCAACGTGGTGACAGTGTCGTCCTTTACGCACCGAATCCCTTTATCATTGAACTAGTAGAAGAGCGATACTTAGGACGTCTTCGGGAATTGTTATCCTATTTTTCAGGAATACGTGAAGTAGTCCTTGCAATTGG

Does anyone knows how to do it with a perl/python script or bioinformatic software?

[finswimmer]

Hello,

how is the order of the sequence files that should be concatenate determined? Sorted by filename? Manual order?

Why do you like to do this?

fin swimmer

[shenwei356]

It depends on the user.

[finswimmer]

Yes, I know. But I'm not sure whether the OP knows that. That's because I'm asking.

Answer 1

[shenwei356]

https://bioinf.shenwei.me/seqkit/usage/#concat

seqkit concat *.fasta > result.fa

Answer 2

[harish]

Assuming you have all the sequences in the same order in multiple files, you can probably do something like:

paste file1 file2 file3 | sed 's/\t>.*//g' | tr -d '\t' > concat.fa

The "sed" part is used for removing the fasta headers after the first tab generated by the paste command and "tr" is used to remove the tabs.

However if the sequences aren't in the same order then you'll have to do some file manipulation.

[fec2]

Hi,

I have the same problem and the sequences aren't in the same order. May I know what should I do? Thank you.

[shenwei356]

Just try seqkit ... The orders do not matter.

Answer 3

[finswimmer]

I like seqkit. But here also an awk solution:

$ cat *.fa|awk -v RS=">" -v FS="\n" -v OFS="\n" '$0 {seq[$1] = seq[$1]$2}; END {for(id in seq){print ">"id, seq[id]}}'

fin swimmer

[fec2]

Hi, may I know can this awk script be apply in my case, where the sequence ID slightly different?

[finswimmer]

Ah, now I see the difference to your question here. I will reopen it, as this difference is important. Let's discuss there.

I also deleted your posts here to keep the thread focused on the OP's problem description.

fin swimmer