A way to verify that GFF and Fasta files make a valid pair (came from the same project version)
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6.3 years ago
Denis ▴ 310

Sorry for the weird question. I have GFF and Fasta files. I'm looking for the way to check if they relate to the same project version, i.e. in the GFF file i have correct annotation for the sequences in Fasta file. Is it realistic task?

assembly genome • 1.9k views
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I think it is easy to spot if a fasta / gff pair are not compatible (different chromosome names, features outside chromosome ranges). However, there is no guarantee the gff and fasta they are from the same project if you don't find incompatibilities.

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One additional note: You'll make your and your colleagues life easier in the future if you include the sequence part in the gff directly. Any gff can contain an arbitrary sized sequence section after a ##FASTA directive See other syntax section in the gff specification

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