Differential expression analysis of normalized expression of RNA seq data

Question

[Javad]

Dear All,

I have a normalized expression table of RNA seq data. I normally use DESeq2 but it needs raw count table not normalized data. Which R package I can use to analyse a Normalised table?

Best,

[ATpoint]

Normalized by what method? Are there replicates?

[ejm32]

This post from the bioconductor support site maybe of help: https://support.bioconductor.org/p/56275/

Answer 1

[Benn]

None. There is no package that is able to do (sound) statistical analysis of normalized RNAseq data. Get read count data, and do what you normally do with DEseq2 or limma voom, etc.