Error displaying GTEX like boxplot in UCSC Genome Browser - "Error creating boxplot from sample data"

Question

[AB]

Hi,

I'm trying to create a GTEx-like barChart and boxplot in UCSC Genome Browser for my data. I followed the instructions on the UCSC help page. The trackhub loads without an error. I can see the barChart in the browser. However, if I click on the barChart, I'm not getting the boxplot. Instead I get an error that says Error creating boxplot from sample data. I dont know what the error is. Can someone help me with this? I'm posting the header of my matrix and category text files.

  matrix.txt

  Name  Samp1   Samp2   Samp3   Samp4   Samp5    
  Sox17 0   0   0   0   0   
  Mrpl15    0   0   0   0   0   
  Lypla1    1.7074123012126 1.59300264994819    0   0   0   
  Tcea1 1.7074123012126 0   0   0   0   
  Rgs20 0   0   0   0   0   
  Atp6v1h   0   0   0   0   0   
  Rb1cc1    0   0   0   0   0   
  4732440D04Rik 0   0   0   0   0

/

categories.txt

samp1  WT
samp2  WT
samp3  Treatment1
samp4  Treatment1
samp5  Treatment2

Answer 1

[AB]

I emailed this issue to UCSC and I heard back from them almost immediately. It was a very silly error. I'm posting this here just in case someone has a similar error

This was the line from my trackDb file

Track scRNA-Seq superTrack on show shortLabel scRNA-Seq longLabel This track contains expression data from single cell RNA-seq

track data
type bigBarChart
visibility full
shortLabel  scRNA-seq
longLabel Expression data from single cell RNA-seq
parent scRNA-Seq on
barChartBars Cluster0 Cluster1 Cluster2 Cluster3 Cluster4 Cluster5 Cluster6 Cluster7 Cluster8
barChartColors #AAAAFF #64B2CE #DA5724 #CBD588 #673770 #D3D93E #38333E #6DDE88 #599861
barChartLabel Clusters
barChartMetric median
barChartUnit Normalized UMI
bigDataUrl http://165.122.23.6/ucsc/JZ_scrna/new/data.bigBed
barChartMatrixUrl http://165.122.23.6/ucsc/JZ_scrna/new/matrix.txt
barChartSampleUrl http://165.122.23.6/ucsc/JZ_scrna/new/categories.txt

barChartUnit has 2 words "Normalized UMI". Making it a single word "Normalized_UMI" fixed the issue