Protein blastp for "QKRAA" Sequence and further Evolution Analysis

Question

[Shicheng Guo]

Hi All,

Aim: blastp to 'QKRAA' and collect all the microbiology and then build the evolution tree.

Any tutorial video to show step by step？

Thanks

[GenoMax]

Is this an assignment question?

blastp to 'QKRAA' and collect all the microbiology and then build the evolution tree.

How does one blastp to such a short sequence and hope to get any useful results?

[Michael]

QKRAA: https://www.ncbi.nlm.nih.gov/pubmed/9109425

The amino acid motif QKRAA, when expressed on HLA-DRB1, carries susceptibility to develop rheumatoid arthritis. This motif is the basis of strong B and T cell epitopes. Furthermore, it is highly overrepresented in protein databases, suggesting that it carries a function of its own. To identify this function, we used QKRAA peptide affinity columns to screen total protein extracts from Escherichia coli. We found that DnaK, the E. coli 70-kD heat shock protein, binds QKRAA. Of interest, DnaK has a natural ligand, DnaJ, that contains a QKRAA motif. We found that QKRAA-containing peptides inhibit the binding of DnaK to DnaJ. Furthermore, rabbit antibody to the QKRAA motif can inhibit binding of DnaJ to DnaK. These data suggest that QKRAA mediates the binding of E. coli chaperone DnaJ to its partner chaperone DnaK.