Mapping and Variant calling

Question

[ttananthi97]

I need to do mapping and variant calling , so I need fastq files and reference fasta file and I don't know how to take and where to take the sequence files(disease related to human). Is there any reference? Because I am new to this.

[Santosh Anand]

https://github.com/ekg/alignment-and-variant-calling-tutorial

Answer 1

[lakhujanivijay]

It looks like you are working or trying to work with human samples. For variant calling, first you should have some samples in your hand; either you generate NGS data for your own samples or you may be interested to work with already available databases like SRA.

check this one out

How to download all searched SRA result data at once?