Entering edit mode
6.1 years ago
nivya.james2016
•
0
Hi all
I'm used the following mapper script for miRNA alignment in miRdeep2. I am getting error and also the reads_vs_genome.arf is empty. I am posting the error message herewith
mapper.pl trim_3_SRR7189567.fastq -e -j -k TCGTATGCCGTCTTCTGCTTGT -l 18 -m -p /home/shanthi/Desktop/Nivya/indexed_genome/genome.fa -s collapsed_reads.fa -t collapsed_reads_vs_genome.arf -v -n -h
parsing fastq to fasta format
discarding sequences with non-canonical letters
clipping 3' adapters
discarding short reads
collapsing reads
mapping reads to genome index
usage: /home/shanthi/miniconda2/bin/convert_bowtie_output.pl reads_mapped.bwt
trimming unmapped nts in the 3' ends
Log file for this run is in mapper_logs and called mapper.log_28941
Mapping statistics
#desc total mapped unmapped %mapped %unmapped
Use of uninitialized value $count2 in subtraction (-) at /home/shanthi/miniconda2/bin/mapper.pl line 708.
total: 2716769 Use of uninitialized value $count2 in print at /home/shanthi/miniconda2/bin/mapper.pl line 708.
2716769 Use of uninitialized value $count2 in division (/) at /home/shanthi/miniconda2/bin/mapper.pl line 709.
Use of uninitialized value $count2 in division (/) at /home/shanthi/miniconda2/bin/mapper.pl line 709.
0.000 1.000
Use of uninitialized value in subtraction (-) at /home/shanthi/miniconda2/bin/mapper.pl line 711.
seq: 2716769 Use of uninitialized value in print at /home/shanthi/miniconda2/bin/mapper.pl line 711.
2716769 Use of uninitialized value in division (/) at /home/shanthi/miniconda2/bin/mapper.pl line 712.
Use of uninitialized value in division (/) at /home/shanthi/miniconda2/bin/mapper.pl line 712.
0.000 1.000
Could somebody help me??
Thank You in advance