Can Orthofinder produce FastA?
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6.1 years ago
bsp017 ▴ 50

Is it possible to get a FastA file of orthologs using Orthofinder? I ran orthofinder (v2.2.7) like this:

orthofinder.py -f sane_files/ -t 40 -M msa

I got around 9000 orthogroups from my genomes. I would like to Blast these against some databases e.g. CAZy database. But I need a FastA file.

Thanks,

James

Orthofinder CAZy sequence alignment • 1.5k views
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Would the following option be relevant in your case?

os               Stop after writing sequence files for orthogroups
                   (requires '-M msa')
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Adding that command gives the proteins that make up the orthogroup. I was wanting a single consensus sequence for each orthogroup. Not sure if there is an easy way to do that? For example, I got 9000 orthogroups, so I wanted a FastA file with 9000 proteins.

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